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. 2011 Sep 20;6(9):e24622. doi: 10.1371/journal.pone.0024622

Figure 1. Th17-related cytokine transcripts in PIOL.

Figure 1

(A, B) A20.IIA-GFP cells (1×104) were inoculated into BALB/c mice on day 0. The mice were sacrificed on day 19. Analysis of HPRT, GFP, IL-17A, IL-21 and IL-22 mRNA expression by RT-PCR of uninjected eyes, PBS-injected eyes, PIOL eyes, and A20.IIA-GFP cells. (A) Representative gel migration from 4 different mice. (B) Densitometry analysis of the gel migration showing mean data from the 4 mice, representative of 2 different experiments. Each data is normalized to HPRT expression. Statistical analyses (Mann-Whitney tests) compare results from d19 PIOL eyes and d19 PBS eyes (*, p<.05). Error bars represent SEM.