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. 2011 Sep 20;6(9):e24655. doi: 10.1371/journal.pone.0024655

Figure 6. Dimer formation by the ARTS CTD peptide.

Figure 6

A and B: NMR analysis of the oxidized versus non-oxidized ARTS 266–274. Overlay of the TOCSY spectra of the fingerprint region (A) showing splitting of the Hβ Cys273 into two signals, coupled with deviation of the chemical shift (B) of the neighboring residues H274, G 272 and Q271. C: Binding of the dimeric fl-ARTS 266–274 to Bir3 using fluorescence spectroscopy. D: Analytical size exclusion chromatography of ARTS CTD with (Blue) and without (red) 2 mM DTT. In the absence of a reducing agent a second peak of ARTS dimer was present.