Figure 5. Expression of peptide b11 inhibits Stat5 phosphorylation.

Phospho-Stat5 Tyr⁶⁹⁴ levels in Ba/F3 cells transduced with peptide b11 or PLC-β3-CT were measured by immunoblotting (A) and fluorescence microscopy (B) with anti-phospho-Stat5-Tyr⁶⁹⁴. (A) Stat5 amounts were measured by reprobing. Phospho-Stat3-Tyr708 and Stat5 amounts were also measured. (B) Nuclei were stained with DAPI (blue). The patterns of phospho-Stat5 were categorized into four (I-IV) and their distributions are shown by scoring ≥200 cells.