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. 2011 Sep 20;6(9):e25040. doi: 10.1371/journal.pone.0025040

Figure 4. The effect of ZMIZ1 on shorter poly-Q AR is insensitive to androgen antagonist.

Figure 4

DU145 cells were transfected with a luciferase reporter driven by the human PSA promoter (100 ng), pcDNA3-β-gal (25 ng), 5 ng of ARQ9, Q24, or Q35, and where indicated, with 10 ng of pcDNA3 empty vector or pcDNA3-FLAG-ZMIZ1. Twenty-four hours after transfection, cells were incubated with different androgen antagonist, including 100 nM of Bicalutamide or Flutamide for 24 h. Cell lysates were prepared for luciferase and β-gal assays. The transfection experiments were repeated three times in triplicate. Relative luciferase units (RLUs) were determined from three independent transfections and are presented as the mean ± SEM of the triplicate transfections. Statistical analyses showed significant differences (p<0.05) between the samples co-expressed with different AR polyQ and ZMIZ1 proteins in the presence of anti-androgens, “*”.