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. 2011 Sep 20;6(9):e25007. doi: 10.1371/journal.pone.0025007

Figure 2. SPR analysis of the binding specificities for Gal-1, -3 CRD and mutants to immobilized GM1-biotin pentasaccharide at 298 K.

Figure 2

(A) The binding curves measured at different concentrations of Gal-3 CRD and the steady state affinity to biotin labeled GM1 pentasaccharide. (B) The sensorgram response profiles for Gal-1 at different concentrations show no interaction between Gal-1 and GM1-biotin. (C) The sensorgram curves and steady state analysis illustrate the mutant Gal-3-g1-L4 with lower affinity to GM1-biotin. (D) The measured curves show the mutant Gal-1-g3-L4 acquires the binding ability to GM1-biotin.