Table 1. Summary data of measured parameters for Gal-1, -3 and mutants interacting with TF antigen or TF-derivatives from ITC and SPR assays at 298 K.
| ITC | SPR | ||||
| Kd (µM) | -ΔH (kcal/mol) | ΔS (cal/mol/deg) | N, stochiometry | Kd (µM) | |
| Gal-1- TF antigen | 4000 | 26.3 | −77.2 | 1.00 | |
| Gal-1- TFN | No affinity | ||||
| Gal-1- GM1 | No affinity | No affinity | |||
| Gal-1-g3-L4-TFN | 719 | 1.5 | 9.4 | 1.00 | |
| Gal-1-g3-L4-GM1 | 568 | 10.3 | −19.8 | 1.00 | Low affinity |
| Gal-3-TF antigen | 47 | 1.9 | 13.5 | 1.04 | |
| Gal-3-TFN | 65 (100%) | 4.6 | 3.8 | 1.21 | |
| Gal-3-GM1 | 57 (100%) | 4.0 | 6.0 | 1.06 | 62 (100%) |
| Gal-3-g1-L4-TFN | 543 (12%) | 3.9 | 1.9 | 1.00 | |
| Gal-3-g1-L4-GM1 | 415 (14%) | 3.2 | 4.7 | 1.00 | 397 (16%) |
Errors in Kd range from 4% to 12%. Errors in ΔH are less than 2%. In the assays of TF titrating Gal-1 and two mutants, the stochiometry is fixed with a value of 1; in other measurements, errors in N value are less than 8%. Square distance numbers came from fitting procedure.