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3T3 cells were transiently transfected with GbX fusion constructs C-terminally tagged with GFP. GFP: EGFP vector without GbX, GbX: full length GbX, GbXΔC: C-terminally truncated GbX, GbX-G2A: non-myristoylatable mutant, GbX-C3S: non-palmitoylatable mutant, GbX-AS: non-acylatable mutant. Insets: Higher magnification of membrane-bound GbX.
