Table 1.
Primer and Taqman probe sequences used for qPCR.
| Subunit | Oligonucleotide | Sequence 5′-3′ |
|---|---|---|
| NaV1.1 | Forward primer | CTGGCAGAAACCCTAACTATGGT |
| Reverse primer | TCAGTCGAAACAGGGACAGAAATG | |
| Taqman probe | CCCAGCTGAAGGTGTC | |
| NaV1.2 | Forward primer | GCTCTGCTTTGTGGGAACAG |
| Reverse primer | GCCTTCACACAGATGTATCCTTCT | |
| Taqman probe | CACTGTCCAGCATCCG | |
| NaV1.3 | Forward primer | CCTCTCAGACCTCGCGGACA |
| Reverse primer | ACGGCAGGTCCATCTGGATT | |
| Taqman probe | CTCCGGCCCTCTTAGAATCCCCAAACC | |
| NaV1.4 | Forward primer | AATGCTAGAACTCAGCACACGGG |
| Reverse primer | CTTGCTTTCAGGCAATGAGAAGAC | |
| Taqman probe | AAGCCAAGCCTGGTGAGCCTTCTCACT | |
| NaV1.5 | Forward primer | GGCTGAGGGAAGAGAGGGC |
| Reverse primer | GGGAGCCTGGGAAATGGAG | |
| Taqman probe | AGCTGCCCACAGCTGGACACAGTTCAG | |
| NaV1.6 | Forward primer | CGTGATGATCCTGACAGTGTTCTG |
| Reverse primer | TGAAGAGCTGCAGGCCAAT | |
| Taqman probe | CAGGGCGAAAACACTC | |
| NaV1.7 | Forward primer | TGACAGCCTGTGAAGGTTGACTC |
| Reverse primer | GCCATGTGTAAATGCTGCCC | |
| Taqman probe | AGGCAGCACAGCCATTAGCTCTGATCC | |
| NaV1.8 | Forward primer | CCTGTCCATTGGGAGTCTGC |
| Reverse primer | GAAGAGGAGGAGGCCGATGT | |
| Taqman probe | CTGCTCTTCGCCCTCATGATGTCCCT | |
| NaV1.9 | Forward primer | AAGCCTTTGTGTTCGACCTGG |
| Reverse primer | ACATCTTTGGGCTGGTCGG | |
| Taqman probe | TCACAAGCCAGGTCTTTGACGTCATCA | |
| GAPDH | Forward primer | GAACATCATCCCTGCATCCA |
| Reverse primer | CCAGTGAGCTTCCCGTTCA | |
| Taqman probe | CTTGCCCACAGCCTTGGCAGC | |
| Cyclophilin A | Forward primer | TATCTGCACTGCCAAGACTGA |
| Reverse primer | CCACAATGCTCATGCCTTCTTTCA | |
| Taqman probe | CCAAAGACCACATGCTTGCCATCCA | |
| β-actin | Forward primer | GAGCTATGAGCTGCCTGAC |
| Reverse primer | AGTTTCATGGATGCCACAGGA | |
| Taqman probe | CATCACTATCGGCAATGAGCGGTTCC |