Fig. 6.

Effect of oral administration of jellyfish collagen extracts on Ig production by splenocytes from OVA-challenged mice. Female 6-week-old BALB/c mice (6 animals per group) were orally administered 10 mg·kg body weight⁻¹·day⁻¹ of jellyfish collagen extracts or 10 mM NaPB for 26 days, and immunized with OVA-alum on days 7 and 21. Splenocytes were harvested on day 26, inoculated in 5% FBS-ERDF medium at 1.0 × 10⁶ cells mL⁻¹ and cultured for 48 h. The amounts of total Igs (a) and OVA-specific Igs (b) in the culture media were measured using ELISA. Solid bars and open bars show the Ig production activity of splenocytes obtained from each mouse administered the vehicle control (VC) and jellyfish collagen extracts (JC), respectively. The results are presented as mean ± SD (n = 6). Asterisks indicate that the data are significantly different from the control data. ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001