Table 1.

Characteristics of V_L T-cell epitopes from U266 and primary B-cell tumors

Tumor source	Peptide	Variable Regiona	Subfamilyb	start positionc	Sequenced	Length	Predictive bindinge	Mean Fluorescencef	shared tumorsg
U266 Myeloma	P19	FR1	IGLV1	5	TQPPSTSET	9	0.76	0.40	1/90
	P20	FR2	IGLV1	39	HLPGKAPKL	9	0.73	0.26	0/90
	P21	FR3	IGLV1	71	SASLAISGL	9	0.68	0.15	12/90
	P23	CDR1	IGLV1	26	TSNIGSNSV	9	0.45	0.24	0/90
	P25	CDR1	IGLV1	32	NSVNWYQHL	9	0.21	1.29	1/90
	P26	CDR3	IGLV1	90	ASWDDRLNGL	10	10.92	0.26	0/90
	P27	CDR1	IGLV1	9	STSETPGQGV	10	3.96	0.53	0/90
	P28	FR1	IGLV1	17	GVTISCSGST	10	0.09	0.15	0/90
	P29	FR1	IGLV1	11	SETPGQGVTI	10	0.20	0.45	0/90
Human CLL1	L50	FR1	IGLV2	29	SVSGSPGQSI	10	0.913	0.07	7/90
Human FL1	L53	FR1	IGLV1	28	SASATPGQRV	10	0.96	0.07	2/90
Human FL2	L54	FR1	IGLV4	29	SASASLGASV	10	0.96	0.06	2/90
Human FL3	L61	FR1	IGLV1	16	KVTISCSGST	10	0.29	0.06	2/90
Human PL1	K18	CDR2	IGKV3	50	LIYGTSSRA	9	3.7	0.52	1/123
								Lambda	27/90
								kappa	1/123

^a,b,cAnalysis and blast by IMGT website (http://imgt.cines.fr/IMGT_vquest/vquest?livret=0&Option=humanIg)

^dSomatically mutated amino acids are bolded

^eEstimate of half-time of disassociation and calculated score in arbitrary units.

^fMean fluorescence Index = (Mean fluorescence with peptide-mean fluorescence without peptide)/(mean fluorescence without peptide).

^gIndividual peptide sequences were compared with Ig V-region sequences available from either 90 FL patients with lambda or 123 patients with kappa L-chain isotype, respectively.

CLL, chronic lymphocytic leukemia; FL, follicular lymphoma; PL, plasma cell leukemia