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. Author manuscript; available in PMC: 2012 Sep 26.
Published in final edited form as: Brain Res. 2011 Jul 28;1414:1–9. doi: 10.1016/j.brainres.2011.07.046

Figure 4. Dual-labeling immunofluorescence photomicrographs depicting CB1R-induced c-Fos expression in catecholaminergic neurons in the RVLM.

Figure 4

Confocal dual-channel images showing tyrosine hydroxylase immunoreactive (TH-ir) neurons (green) and c-Fos immunoreactive (Fos-ir) cell nuclei (red) in RVLM of rats treated as described under methods with (A) vehicle, (B) 15 μg WIN55,212-2, (C) AM251 + 15 μg WIN55,212-2 and (D) Muscimol + 15 μg WIN55,212-2. White or yellow arrowheads indicate single labeled TH-ir neurons or c-Fos-ir cell nuclei, respectively. White arrows denote c-Fos/TH co-labeled cells. Scale bar, 20 μm.