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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Muscle Res Cell Motil. 2011 Jun 5;32(2):65–76. doi: 10.1007/s10974-011-9252-y

Fig. 2.

Fig. 2

Changes in protein expression after 7 days of organ-culture and the effect of 10 µM Y-27632 in rabbit mesenteric artery. A Representative immunoblot images of various proteins from freshly isolated (D0; Fresh) and 7 days cultured arteries in FBS-free medium (D7; No FBS), and FBS-supplemented medium in the absence (D7; FBS) and presence of Y-27632 (D7; FBS + Y-27632). Total actin (pan-actin) contents under different conditions were matched. For actins, protein extracts diluted ten-fold were loaded. B and C Average expression levels of contractile/regulatory proteins (n = 4–6). D Representative RT–PCR images (upper) and a quantitative summary of eNOS mRNA expression (lower, n = 4). The symbols for statistical significance are defined as described in the legend of Fig. 1