Fig. 3.

Effect of 10 µM Y-27632 pretreatment during FBS-supplemented organ-culture for 7 days on agonist-induced contractions (A) and ACh-induced relaxation (B) in rabbit mesenteric arterial rings. A Comparison of steady tonic level of contractions induced by various stimulants (124 mM K⁺, 30 µM PE and 0.3 µM ET-1) in arteries pretreated under various organ culture conditions (n = 9). In response to 124 mM K⁺, fresh arteries produced a transient contraction followed by a reduction to a low level while monotonic contraction was seen at D7 in organ-cultured arteries irrespective of pretreatment with FBS and Y-27632. B Summary of relative 100 µM ACh and 10 µM sodium nitroprusside (SNP)-induced relaxation at steady state (n = 6–9). Relative relaxation was normalized with that of the D0;Fresh. The symbols for statistical significance are defined as described in the legend of Fig. 1