Figure 2. Spheroid formation of hepatoma subpopulations after FACS enrichment.
A, B and C: Spheroid cores were formed after seeding on low-attachable plates for 3 weeks, and co-stained with antibodies against AFP and CD133 or PCNA. D. Summary of spheroid formation in Hep3B and Huh-7 subpopulations 3 weeks after FACS enrichment.