Figure 5. Acquisition of EMT in CD133−/ALDHlowHep3B, HLE and HLF cells.
FACS-enriched CD133+/ALDHhigh or CD133−/ALDHlow subpopulations were stained with either Cy5-conjugated monoclonal antibodies against E-cadherin or vimentin in red. Cy5-stained images were overlaid with DAPI nuclear counter-staining in blue. Hep3B and HLF (20×), HLE (10×).