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. Author manuscript; available in PMC: 2012 Sep 16.
Published in final edited form as: ACS Chem Biol. 2011 Jul 20;6(9):952–961. doi: 10.1021/cb200205z

Figure 6.

Figure 6

DMH1 inhibits sheet migration, but not SMA expression, in proepicardial explants. HH stage 16 proepicardia were excised from chick embryos and incubated for 24 hours. a,d) Proepicardia incubated in medium exhibited a uniformly spread epicardial cell sheet with SMA expression at the distal edge and ZO-1, marking epithelial cells, in the interior of the sheet. b,e,g) Proepicardia cultured in TGFβ1 had upregulated SMA expression centrally and peripherally in the outgrowths, but TGFβ1 treatment did not increase outgrowth migration rate from medium controls. c,f,g,j) Proepicaria stimulated with TGFβ1 and treated by DM/LDN-193189 had reduced epicardial sheet migration and SMA expression. k) Proepicardia stimulated by TGFβ1 and treated with DMH1 inhibited outgrowth migration, but had enhanced SMA expression and diminished zonula occludins-1 protein expression throughout the epicardial sheet. [Measurements assessed per condition=40; Scale bars=200µm for A–C and 50µm for D–F; (*p<0.001; n≥5 proepicardia); ZO-1=Zonula Occludins-1].