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PMF for hydration in CAVER channels 2e (a), W2 (b), 2d (c), 2f (d), S (e), W1 (f), and 2a (g) for apo-P450cam (dark gray/blue) and camphor-bound P450cam (light gray/red). Five different reaction-coordinate bin sizes (1.0–3.0 Å) were used and PMF profiles were smoothed with B-spline curves.
