Figure 5.

Dimerization enthalpy of WGA at pH 3 (50 mM Gly/HCl, 0.1 M NaCl) as a function of temperature. The solid line represents the best fitting of a third-order polynomial, which yields increasing ΔCp_A from −0.8 kcal mol⁻¹ K⁻¹ at 33°C to −5.5 kcal mol⁻¹ K⁻¹ at 46°C. The dotted line represents the best fitting of a straight line to data in the 30–35°C temperature range, with ΔCp_A = −0.8 kcal mol⁻¹ K⁻¹.