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Unfolding parameters for the calorimetric transitions of monomeric WGA, at pH 2 and varying concentrations of GndHCl
| Transition |
|||
|---|---|---|---|
| 1 | 2 | 3 | |
| 0 M GndHCl | |||
| Tmi (°C) | 68.0 ± 0.7 | 79.0 ± 0.6 | 91.1 ± 0.4 |
| ΔHUi (kcal/mol) | 31 ± 2 | 43 ± 3 | 42 ± 1 |
| 1.5 M GndHCl | |||
| Tmi (°C) | 55.2 ± 0.2 | 69.4 ± 0.1 | 86.0 ± 0.3 |
| ΔHUi (kcal/mol) | 29 ± 1 | 40 ± 2 | 39 ± 1 |
| 2 M GndHCl | |||
| Tmi (°C) | 52.6 ± 0.5 | 67.9 ± 0.3 | 81.9 ± 0.3 |
| ΔHUi (kcal/mol) | 28 ± 1 | 38 ± 1 | 36 ± 1 |
