Figure 6.

Experimental and computational sensitivity of hESC^ChR2-CM with respect to light intensity. With light on, the photocurrent I_ChR2 increases rapidly, peaks, and decays toward a characteristic plateau value. With light off, the photocurrent I_ChR2 drops rapidly and decays to zero. Light intensity is varied from 12.5% to 25%, 50%, and 100% (top). Whole-cell voltage-clamp reveals an increased photocurrent I_ChR2 as the light intensity increases (middle). The computational hESC^ChR2-CM model captures the light sensitivity and displays increased photocurrents I_ChR2 with increased light intensity (bottom).