Skip to main content
. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Pediatr Surg. 2011 Sep;46(9):1711–1719. doi: 10.1016/j.jpedsurg.2011.01.023

Figure 1. Fgfr2IIIbrec/rec mutants do not express the Fgfr2IIIb exon.

Figure 1

RT-PCR was used to assess expression of the Fgfr2IIIb exon in wild-type embryos and embryos homozygous for the Fgfr2IIIbrec allele. RNA was isolated from whole embryos at E18.5 and converted to cDNA. RT-PCR was performed with primers that hybridize within exon IIIb of Fgfr2 (A) or with primers for Gapdh (B). The product size for the Fgfr2IIIb exon is 102 bp and 496 bp for Gapdh. Note the lack of expression of the Fgfr2IIIb exon in the embryos homozygous for the Fgfr2IIIbrec allele.