Fig. 4.

(A) Schematic of the inserts for Late Cherry Early EGFP (LREG) and Late Cherry Early Venus (LREV) multi-reporter viruses. There are no intervening sequences between promoter and reporter elements. Inserts were placed between the Vaccinia J4R and J5L genes, as shown in Fig. 1. (B) EGFP and Cherry fluorescence in a focus of Vero cells 48 hpi following low MOI LREG infection. 10× magnification. (C) Schematic of the inserts for Intermediate Cherry, Early Venus, and Late Tag-BFP in the multi-reporter Triple Virus (TrpV). Intergenic insertion sites are noted (J4/J5 and K7/F1). (E) Venus, Cherry, and Tag-BFP fluorescence in two foci of A549 cells 24 hpi following low MOI TrpV infection. 10× magnification.