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Nickel-dependent regulation of the NikR target genes in H. pylori WT 26695 and the isogenic ΔnikR mutant. Expression of the NikR-upregulated genes ureA, hpn, hpn2, hspA (A) and NikR-downregulated genes nixA, fur, exbB, frpB4, fecA3, hydB, nikR (B) was measured by qRT–PCR. The level of transcripts after overnight culture in BBβ without added NiCl2 or with a supplementation of 10 μM NiCl2 was determined relative to that of the level of ppk (hp1010) under the same conditions.
