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Effects of increasing concentrations of nickel on the transcription of NikR target genes in H. pylori WT 26695 strain. Expression of the NikR-upregulated genes ureA, hpn, hpn2, hspA (A) and NikR-downregulated genes nixA, fur, exbB, frpB4, fecA3, hydB, nikR (B) was measured by qRT–PCR. The levels of transcripts were quantified after overnight culture in BBβ supplemented with 0, 5, 10 or 20 µM NiCl2. The housekeeping gene ppk (hp1010) was used as a reference.
