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Western blots of soluble proteins extracted from overnight grown H. pylori WT 26695 strain and the isogenic Δnik R mutant in response to increasing concentrations of supplemented NiCl2 (0, 5 or 10 µM NiCl2). Targeted proteins are UreA, NikR (A), ExbB-TAP (B) and NixA-TAP (C). Coomassie-stained gels are shown as loading controls.
