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Western blots of H. pylori WT 26695 and ΔnikR mutant proteins in response to 10 μM NiCl2. Samples were removed at 0, 2, 5 and 10 h after addition of supplemented nickel to exponential phase cultures cells (OD600nm = 0.5). The targeted proteins are UreA, NikR (A) ExbB-TAP (B) and NixA-TAP (C). Coomassie-stained gels are shown as loading controls.
