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. 2011 Jun 25;39(17):7598–7609. doi: 10.1093/nar/gkr484

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — The effect of a moderate secondary structure on the efficiency and fidelity of translation from a strong and TISU AUG contexts located five nt from the m7G cap. (A) Schematic representation of a secondary structure positioned 6 or 15 nt DS of the AUG is shown on the top panel. The constructs with or without secondary structure were in vitro transcribed and then translated in vivo by transfection into 293T cells and translation efficiency was assayed by immunoblot with anti-GFP. Representative immunoblots of three independent experiments is shown. The graphs represent the average ± SD of the intensity of the upstream (30 KDa) and DS (27 KDa) translation site of 3–5 independent experiments (In the case of the strong AUG, the densitometry analysis was done using the blot from the long exposure).