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Recombinant expression of TcdB with a BoNT/A RBD. Recombinant AGT-TcdB, AGT-TcdB-BoNT/A-Hc, and BoNT/A-Hc were expressed and purified. (a) Each preparation containing 250 ng of protein was analyzed by SDS-PAGE and protein stain. (b) The preparations were also analyzed by Western blots using anti-AGT serum, anti-TcdB serum, or anti-BoNT/A-Hc mAb as indicated. The position of molecular weight markers is indicated with arrows.
