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A TcdB chimera with the BoNT/A receptor-binding domain more rapidly elicits Neuro2A cell rounding. Neuro2A cells were exposed to three different concentrations (20 ng/mL, 4 ng/mL, and 0.16 ng/mL) of AGT-TcdB-BoNT/A-Hc or AGT-TcdB and the % cell rounding was assessed hourly for 5 hr. Data shown are representative of 2 independent experiments.
