Table I. The gene target accessions, target sequence, and RHs of CoDA ZFNs that generated mutations in the target genes.
| Gene Namea | Accession No. | Target Site | Spacerb | RH (F1) | RH (F2) | RH (F3) |
| DCL1ac | Glyma03g42290 | cAGCAACCTCTTATAAGAGGGCGTGg | 6 | TKQILGR | HKSSLTR | RHDQLTR |
| gTCGTTGGAGAATATTCTCCCGCACc | SRFTLGR | LKEHLTR | RVDNLPR | |||
| DCL1bc | Glyma19g45060 | cAGCAACCTCTTATAAGAGGGCGTGg | 6 | TKQILGR | HKSSLTR | RHDQLTR |
| gTCGTTGGAGAATATTCTCCCGCACc | SRFTLGR | LKEHLTR | RVDNLPR | |||
| DCL4ac | Glyma17g11240 | tTGCTTCATCACAATGGAGATGATt | 5 | RGQELRR | QQTNLTR | VGSNLTR |
| aACGAAGTAGTGTTACCTCTACTAa | TKQRLVV | VRHNLTR | QTTHLSR | |||
| DCL4bc | Glyma13g22450 | tTGCTTCATCACAATGGAGATGATt | 5 | RGQELRR | QQTNLTR | VGSNLTR |
| aACGAAGTAGTGTTACCTCTACTAa | TKQRLVV | VRHNLTR | QTTHLSR | |||
| RDR6a | Glyma04g07150 | aGGCAACGACATCAGAGGAGTGGAAa | 6 | LKKDLLR | HKSSLTR | DRTPLQR |
| tCCGTTGCTGTAGTCTCCTCACCTTt | HKPNLHR | RREVLEN | QKPHLSR | |||
| RDR6b | Glyma06g07250 | aGGCAACGACATCAGAGATGTGGAAa | 6 | LKKDLLR | HKSSLTR | DRTPLQR |
| tCCGTTGCTGTAGTCTCTACACCTTt | HKPNLHR | RREVLEN | ISHNLAR | |||
| HEN1a | Glyma08g08650 | aGCACGCGACCACGCGTAGACGCAt | 5 | RSRNLTL | RTDTLAR | ESGALRR |
| tCGTGCGCTGGTGCGCATCTGCGTa | EESNLRR | DRGNLTR | QSTSLQR |
a
Gene names are based on homology to previously characterized genes in Arabidopsis.
b
Measured in nucleotides.
c
Represents two duplicate copies (“a” and “b”) that both perfectly match the ZFN target site.