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. 2011 Apr 18;156(2):585–595. doi: 10.1104/pp.111.175976

Figure 4.

Figure 4.

The rol1-2 cell shape phenotype and localization of RHM1-GFP. Adaxial epidermal cells of 6-d-old rol1-2 seedlings lack the jigsaw puzzle-like shape (A), whereas the abaxial cells are wild type like (B). rol1-2 mutants transformed with RHM1:RHM1-GFP showed complementation and revealed fluorescence on the adaxial side of cotyledons (C) and in emerging leaves and trichomes (D). In roots, GFP fluorescence was observed in epidermal cells of the meristematic and elongation zone, whereas in the late elongation zone, RHM1-GFP was detected in all cell layers (E). F shows the differentiation zone where the GFP signal occurred in all tissues and in emerging root hairs (G). DAPI staining of DNA and merging with the GFP data indicate a cytoplasmic localization of RHM1-GFP (H). Bars = 1 mm (C and D), 40 μm (A and B), 50 μm (E and F), and 20 μm (G and H).