Figure 3.
Phenotype and protein gel-blot analyses of the double mutants between fin219 and cop1 alleles under darkness and cFR light. A, Phenotypes of the double mutant fin219-2/cop1 under darkness and cFR conditions. Three-day-old seedlings of wild-type Col, fin219-2, cop1-4, cop1-6, fin219-2/cop1-4, and fin219-2/cop1-6 underwent phenotype examination (n = 30). B, Quantitative analysis of hypocotyl lengths of the seedlings shown in A. Data represent results of at least two replicates (n = 30). C, Protein gel-blot analysis of FIN219, COP1, and HY5 in the double mutant fin219-2/cop1 seedlings grown under darkness and cFR light. Protein extracts were isolated from the seedlings shown in A and used for protein gel-blot analysis. Each lane contained 100 μg of total protein. RPN8 was a loading control. The numbers below each blot represent relative expression ratios normalized to RPN8 expression; the level of wild-type Col was arbitrarily set to 1. Different lowercase letters represent significant differences by ANOVA at P < 0.05. The asterisks indicate artificial bands. The FR fluence rate is 1.47 μmol m−2 s−1. [See online article for color version of this figure.]