Figure 6.

FIN219 can exclude COP1 out of the nuclei into the cytoplasm. A, Subcellular localization of GFP-COP1 in the protoplasts isolated from glucocorticoid-inducible FIN219 (pGR:FIN219) transgenic plants. The transfected protoplasts were cultured on W5 solution containing ethanol (EtOH) or 1 μm Dex and incubated in the dark overnight. The experiments were repeated once and shown in Exp.1 and Exp. 2. B, Percentage of transfected protoplasts with different subcellular locations of GFP-COP1. Thirty transfected protoplasts were counted for the subcellular localizations of GFP-COP1. C, Cytoplasm; N, nuclei; N/C, both locations. C, Protein gel-blot analysis of FIN219, COP1, and HY5 levels in the protoplasts with (+Dex) or without (EtOH) Dex treatment. The asterisk indicates a nonspecific band. RPN8 was a loading control.