FIN219 shows differential expression levels and regulation on HY5 in fin219 mutants under low and high fluence rates of FR light. A, Phenotypic examination of wild-type Col, fin219-1, fin219-2, and phyA-211 mutant seedlings under LF and HF light conditions. Three-day-old seedlings were used for phenotypic examination. The bottom panel shows a closeup image of cotyledons and anthocyanin accumulation. LF, 1.42 μmol m −2 s−1; HF, 20.54 μmol m−2 s−1. B, Quantitative analysis of hypocotyl lengths of the seedlings shown in A (n = 30). C, Quantitative measurement of anthocyanin accumulation of the seedlings shown in A (n = 6). D, Protein gel-blot analysis of Col, fin219-1, fin219-2, and phyA-211 seedlings shown in A. The asterisk indicates artificial bands. RPN8, a subunit of the regulatory complex of the 26S proteasome, was a loading control. The number below each blot represents the relative level of the indicated protein. The level in wild-type Col was arbitrarily set to 1. Different lowercase letters represents significant differences by ANOVA at P < 0.05.