Figure 5.

Complementation rescued the CCM deficiency in cia6. By transforming genomic DNA containing the wild-type CIA6 gene into the cia6 mutant, the resulting complemented strain cia6-comp exhibited a growth phenotype similar to the wild type (Fig. 1). A, The rate of photosynthesis as a function of the DIC was measured using low-CO₂-grown D66 (green circles), cia6 (black triangles), cia5 (red triangles), and the complemented strain cia6-comp (blue squares). B, RT-PCR analysis of D66, cia6, cia5, and the complemented cia6 (cia6-comp) using RNA isolated from low-CO₂-grown cells as templates for RT. Primers were designed to amplify the entire ORF. The reappearance of the CIA6 full-length ORF in the complemented strain (cia6-comp) was observed on a DNA agarose gel. The last lane contained the no-RNA control. [See online article for color version of this figure.]