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Relax–self and active–rigor transition work histograms contrast continuous and discretized work probabilities. ΦRe–Re’ s from Figure 11A are used in eq 4 to compute the relax–self work histogram. It features a probability spike at zero work that rises to ~0.2 (not shown). Relaxed cross-bridges are not (or rarely) actin bound and are not discritized. The active–rigor transition work histogram is identical to that in Figure 12.
