Fig. 5.

Severity of unc-5(e53) axon pathfinding defects is mitigated by loss of unc-40. (A) Percentage of VD axons that have pathfinding errors by the time they reach the dorsal edge of the ventral muscle quadrant. Error bars represent 2× standard error of the mean; asterisks indicate a significant difference between wild type and the mutant (P<0.01); + indicates a significant difference between unc-5(e53) alone and the unc-5(e53); unc-40(n324) double mutant (P<0.01) determined by two-sided t-test with unequal variance. (B,C) Representative images showing VD axons (arrows) with mCherry expression (red) after their complete outgrowth in L4 animals. Ventral and dorsal muscle quadrants with CFP expression are blue. In unc-5(e53) most axons do not advance beyond the ventral muscle quadrants, whereas in unc-5(e53); unc-40(n324), many axons extend past the ventral quadrants. Scale bar: 5 μm.