Fig. 5.

Loss of astrocytic FN impairs VEGFR2-mediated stimulation of migration via the PI3K pathway. (A) Western blot analysis showed reduced phosphorylation of VEGFR2 but not of total VEGFR2 protein levels in retinas of P5 FN^ACko compared with control mice. (B) PI3K activity determined by pull-down assays with pY-peptides was markedly reduced in FN^ACko retinas. Control and FN^ACko retinas for p-peptide, n=7. ^*P<0.05. Values represent mean+s.e.m. (C) Phosphorylation of serine residue 473 (pSer) of AKT relative to total levels of AKT was significantly reduced in FN^ACko retinas (C) but not in Itga5^ECko retinas (C). (D) Densitometric analyses of P5 control (n=4) and Itga5 retinas (n=5). Values represent mean+s.e.m.