Effect of ROI boundary shifts on mean regional Fgas. (A–D) ROIs used to compute mean Fgas, divided along the ventrodorsal axis (A, sagittal plane), laterolateral axis (B, transverse plane), cephalocaudal axis (C, coronal plane), and combining all directions (D, 3-dimensional view). (E) Mean regional gas fractions for each ROI type in A–D (represented by respective colors) demonstrated small changes in Fgas as result of ROI boundary shifts.