Figure 6.
Effects of 9,10-DiHOME treatment on LVDP recovery, 8-iso-PGF2α levels and coronary resistance in WT hearts. WT hearts were isolated and perfused exactly as shown in Fig. 1A. Starting 10 min prior to ischemia and throughout recovery, hearts were treated with either vehicle (100% EtOH) or 250 nM 9,10-DiHOME. A) Treatment of WT hearts with 9,10-DiHOME results in reduced LVDP and RPP recovery of LVDP at R40 compared to vehicle; n = 9–10/group. B) Treatment of WT hearts with 9,10-DiHOME did not result in increased generation of 8-iso-PGF2α in heart perfusates compared to vehicle; n = 15/group. C) Treatment of WT hearts with 9,10-DiHOME results in increased coronary resistance compared to vehicle; n = 21/group. Values are means ± se. *P < 0.05 vs. vehicle.