Figure 3.

SOD1 deficiency increases the SO level but does not further enhance apoB100 degradation induced by DHA. A) SDS-PAGE was performed on cell lysates from McA cells treated with control siRNA control (Si-Cont), SOD1-siRNA (Si-SOD1), and nontreated cells (NT). Western blots (representative blot is shown) were probed for SOD1 and GAPDH as a loading control. B) Histogram showing total apoB100 recovery after 90 min of chase in presence of BSA or DHA in McA cells treated for 48 h with Si-SOD1 or Si-Cont. C) Histogram showing quantity of 2-OH-E⁺ in McA cells treated for 48h with Si-SOD1 or Si-Cont and then treated with BSA or DHA. D) Lipid peroxidation was measured in the siRNA-treated cell after 3 h in the presence of BSA or DHA. Data are expressed as fold increases of the amount of MDA measured in Si-Cont-treated cells. Data represent means ± se (n=3) from 2 independent experiments. ns, nonsignificant (P>0.05). **P < 0.01.