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. 2011 Oct;25(10):3634–3645. doi: 10.1096/fj.11-186924

Figure 5.

Figure 5.

KLF4 regulates Th17- but not Th1-, Th2-, and Treg-cell differentiation. A) Histograms of Th1, Th2, Th17, and Treg cells from Klf4fl/flCD4-Cre+ (KO) and Klf4fl/flCD4-Cre− (WT) mice. Numbers of Th1, Th2, Th17, and Treg cells were determined by intracellular staining with anti-IFN-γ, anti-IL-4, anti-IL-17 and anti-FOXP3. Gates for each subset were set according to each isotype-matched control Ab staining. B) Mean percentages of positive cells for the indicated Th cytokines from KO and WT mice (n=8). C) No significant difference of IFN-γ concentration in the supernatant of 4 h PMA/ionomycin restimulated Th1-polarized cells from KO mice compared with cells from WT mice (n=8). D) Decreased IL-17 concentration in the supernatant of 4 h PMA/ionomycin restimulated Th17 polarized cells from KO mice compared with cells from WT mice. IFN-γ and IL-17 concentrations were measured by ELISA (n=8). Data are means ± se. *P < 0.05.