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. 2011 Oct;25(10):3465–3476. doi: 10.1096/fj.11-186296

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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/us/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Concentration-k_off relationship of ABA-X-BY630 dissociation in the absence and presence of competitive ligands at the A₃-AR and A₁-AR. Dissociation rate k_off of 30 nM ABA-X-BY630 from CHO-A₃ (A) and CHO-A₁ cells (B) in the absence (□) or presence of XAC (■), NECA (○) and adenosine (●). Values for k_off were derived from global analysis of dissociation kinetic data to a monophasic exponential decay.). Rate of perfusion was maintained at >12 complete fluid exchanges/min. Data points are expressed as means ± se from 3–11 separate experiments; each replicate represents the average of 10 cells.