Figure 3.

Ccl2^−/− mice showed impaired MO/MP recruitment from bone marrow to blood and from blood to injured muscles, less severe than seen in Ccr2^−/− mice. A) Flow cytometry at baseline showed that numbers of 7/4⁺Ly-6G⁻ MOs/MPs (a, b) were increased in bone marrow but reduced in blood (a, c) in Ccl2^−/− mice (n=15), and more so in Ccr2^−/− mice (n=15), as compared with wild-type controls (n=15). B) At d 3 postinjury, numbers of 7/4⁺Ly-6G⁻ MOs/MPs were also increased in bone marrow (a, b) and reduced in blood (a, c) in Ccl2^−/− mice (n=12), and more so in Ccr2^−/− mice (n=13), as compared with wild-type controls (n=15). C) Flow cytometry at d 3 showed that the numbers of F4/80⁺ MOs/MPs were reduced in blood and in injured muscles of Ccl2^−/− mice (n=15) and more severely reduced in Ccr2^−/− mice (n=15) as compared with wild-type controls (n=15), among which the Ly-6C⁺, but not the Ly-6C⁻, subset was significantly reduced in blood (a, b). However, both Ly-6C⁺ and Ly-6C⁻ subsets were significantly reduced in injured muscles of Ccl2^−/− mice and more severely reduced in Ccr2^−/− mice (a, c). *P < 0.05; **P < 0.01; ***P < 0.001.