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. 2011 Oct;25(10):3695–3707. doi: 10.1096/fj.10-178871

Figure 4.

Figure 4.

Res treatment decreases E2-induced signal and ERα mRNA. A) ERE luciferase reporter assay. XETL was transiently transfected into MCF-7 cells treated with vehicle (−) or 10 nM E2 and/or different concentrations of Res. After 18 h, cells were harvested, and luciferase activities were determined. RLU, relative luciferase units. ◊P < 0.05 vs. E2. B) Immunoblot analysis. Cells were treated with vehicle (−) or 20 μM Res at different times as indicated. C) Immunoblot analysis. Cells were treated with vehicle (−) or 10 nM E2 with or without 20 μM Res at different times as indicated. Band intensities were evaluated in terms of optical density arbitrary units and are expressed as percentages of control. ◊P < 0.05 vs. 24 h E2. D) Real-time PCR assay. mRNA expression of ERα. Cells were treated with vehicle (−) or different concentrations of Res and/or OHT (1 μM or 100 nM) for 24 h as indicated. ◊P < 0.05 vs. untreated cells; ◊◊P < 0.05 vs. 1μM OHT. E) Real-time PCR assay. mRNA expression of ERα in MCF-7 cells treated with vehicle (−) or 20 μM Res and/or 10 μg/ml Chx for 24 h. ◊P < 0.01 vs. Res. Results represent means ± sd of 3 independent experiments.