Figure 1.

Pulmonary vascular cells express different AMPKα subunits. A) Immunoblot analysis reveals that the AMPK catalytic α1 (AMPKα1) subunit is selectively expressed by PMVECs (MV). PAECs (PA) express AMPKα2, whereas PASMCs (SM) express AMPKα1 and AMPKα2. Caveolin-1 (Cav-1) was used as a loading control. MW, molecular weight markers. B) AMPKα1, but not AMPKα2, RNA is detected in PMVECs. AMPKα1 and AMPKα2 RNAs are detected in PAECs. RT, with (+) or without (−) reverse transcriptase added to reaction mix.