Figure 5.

PMVEC monolayer repair requires AMPKα1. A) Decreasing AMPKα1 activity, by inhibition (using compound C) or shRNA (Δα1), blocks formation of cell-cell networks in PMVECs (MV), but not PAECs (PA), on Matrigel (middle panels). AMPK activation using AICAR enhances network formation in shRNA-expressing cells, but not in control MV or PA (bottom panels). Micrographs taken 4 h postseeding. B) AMPK inhibition impairs wound resealing in PMVECs. Control monolayers reseal 18 h after wounding (top panels and Supplemental Video S1). Treatment with shRNA (Δα1) to AMPKα1 impairs wound resealing (middle panels and Supplemental Video S2). More complete inhibition of AMPKα1 using compound C produces greater blockade of cell migration and barrier repair (bottom panels and Supplemental Video S3).