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. 2011 Oct;25(10):3356–3365. doi: 10.1096/fj.10-179218

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Figure 7. — AMPKα1 and N-cadherin colocalize and coimmunoprecipitate in PMVECs. A) Confocal micrographs of fluorescently labeled antibodies to AMPKα1 (red, top left panel) and N-cadherin (green, top center panel) show AMPKα1/N-cadherin colocalization (yellow, overlay image, top right panel) at cell-cell borders. AMPKα2 does not localize to cell-cell borders (red, bottom left panel) or colocalize with cadherins in PAECs (green, bottom right panel). B) Coimmunoprecipitation of AMPKα1 and N-cadherin with antibody used for immunoprecipitation (IP) of N-cadherin and immunoblotting (IB) with antibody to AMPKα1 (top panel) or with IP by antibody to AMPKα1 and IB with N-cadherin antibody (center panel). AMPKα1 and VE-cadherin do not coimmunoprecipitate (bottom panel). Mw, molecular weight markers; Wcl, whole-cell lysate. C) Immunoblots of membrane fractions show N-cadherin and AMPKα1 localized to caveolin-1-enriched membranes (fractions 3, 4, and 5). VE-cadherin is primarily found in fraction 7, which is not enriched in caveolin-1, N-cadherin, or AMPKα1. Numbers refer to the buoyancy of the membrane fractions, from 3 (lightest) to 9 (heaviest).