Figure 4.
Differential expression of known and novel miRNAs in normal, uninvolved psoriatic and involved psoriatic skin. (A) Venn diagram indicating the number of differentially expressed miRNAs that were ±2-fold differentially expressed in any of the three comparisons: PP/NN, PP/PN, PN/NN. (B) Heat map showing hierarchical clustering of skin samples on the basis of 98 differentially expressed miRNAs. (C) qRT-PCR levels of differentially expressed miR-135b, miR-431, miR-675* and novel #117/miR-203-AS in 10 NN, 10 PN and 10 PP patients. Lines indicate matched uninvolved and involved samples from the same patient (***P < 0.001, **P < 0.01,*P < 0.05). Relative expression was calculated with respect to the endogenous snoRNA Z30 (see Materials and Methods). NN, normal skin; PN, uninvolved psoriatic skin; PP, involved psoriatic skin.