Figure 1.

Neurofibromin is expressed in osteochondroprogenitor cells and their progeny. (A) Nf1 mRNA expression was detected by RT-PCR in multiple primary cells and cell lines including adherent bone marrow osteochondroprogenitors (BMSCs and C3H10T1/2), chondrocytes (Ch: rib primary chondrocytes and TMC23 cells) and osteoblasts (OB) (calvaria osteoblasts and MC3T3 cells). (B) Neurofibromin expression (brown staining) was detected in growth plate hypertrophic chondrocytes, trabecular osteoblasts and cells of the periosteum and perichondrium (immunohistochemistry).